View source for 6s23


==Crystal structure of ene-reductase GsOYE from Galleria sulphuraria in complex with 2-methyl-cyclopenten-1-one==
<StructureSection load='6s23' size='340' side='right'caption='[[6s23]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6s23]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Galdieria_sulphuraria Galdieria sulphuraria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S23 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=KSW:2-methylcyclopenta-2,4-dien-1-one'>KSW</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s23 OCA], [https://pdbe.org/6s23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s23 RCSB], [https://www.ebi.ac.uk/pdbsum/6s23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s23 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M2XAQ9_GALSU M2XAQ9_GALSU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Looking for new ene-reductases with uncovered features beneficial for biotechnological applications, by mining genomes of photosynthetic extremophile organisms, we identified two new Old Yellow Enzyme homologues: CtOYE, deriving from the cyanobacterium Chroococcidiopsis thermalis, and GsOYE, from the alga Galdieria sulphuraria. Both enzymes were produced and purified with very good yields and displayed catalytic activity on a broad substrate spectrum by reducing alpha,beta-unsaturated ketones, aldehydes, maleimides and nitroalkenes with good to excellent stereoselectivity. Both enzymes prefer NADPH but demonstrate a good acceptance of NADH as cofactor. CtOYE and GsOYE represent robust biocatalysts showing high thermostability, a wide range of pH optimum and good co-solvent tolerance. High resolution X-ray crystal structures of both enzymes have been determined, revealing conserved features of the classical OYE subfamily as well as unique properties, such as a very long loop entering the active site or an additional C-terminal alpha helix in GsOYE. Not surprisingly, the active site of CtOYE and GsOYE structures revealed high affinity toward anions caught from the mother liquor and trapped in the anion hole where electron-withdrawing groups such as carbonyl group are engaged. Ligands (para-hydroxybenzaldehyde and 2-methyl-cyclopenten-1-one) added on purpose to study complexes of GsOYE were detected in the enzyme catalytic cavity, stacking on top of the FMN cofactor, and support the key role of conserved residues and FMN cofactor in the catalysis.

Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.,Robescu MS, Niero M, Hall M, Cendron L, Bergantino E Appl Microbiol Biotechnol. 2020 Jan 13. pii: 10.1007/s00253-019-10287-2. doi:, 10.1007/s00253-019-10287-2. PMID:31930452<ref>PMID:31930452</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6s23" style="background-color:#fffaf0;"></div>

==See Also==
*[[NADPH dehydrogenase|NADPH dehydrogenase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Galdieria sulphuraria]]
[[Category: Large Structures]]
[[Category: Bergantino E]]
[[Category: Cendron L]]
[[Category: Hall M]]
[[Category: Niero M]]
[[Category: Robescu MS]]