View source for 6s6s


==Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.==
<SX load='6s6s' size='340' side='right' viewer='molstar' caption='[[6s6s]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6s6s]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Azospirillum_brasilense Azospirillum brasilense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S6S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6s OCA], [https://pdbe.org/6s6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s6s RCSB], [https://www.ebi.ac.uk/pdbsum/6s6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GLTB_AZOBR GLTB_AZOBR] 
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== Publication Abstract from PubMed ==
Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an alpha-subunit (alphaGltS) and a beta-subunit (betaGltS) that assemble in different alphabeta oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (alpha4beta3, alpha4beta4, alpha6beta4 and alpha6beta6, in the 3.5- to 4.1-A resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S](+1,+2) clusters within betaGltS.

Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.,Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159<ref>PMID:31473159</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6s6s" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glutamate synthase|Glutamate synthase]]
== References ==
<references/>
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[[Category: Azospirillum brasilense]]
[[Category: Large Structures]]
[[Category: Bolognesi M]]
[[Category: Chaves-Sanjuan A]]