View source for 6sib


==Crystal structure of the domain-swapped N-lobe dimer of drosophila Arc 2==
<StructureSection load='6sib' size='340' side='right'caption='[[6sib]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6sib]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SIB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sib OCA], [https://pdbe.org/6sib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sib RCSB], [https://www.ebi.ac.uk/pdbsum/6sib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sib ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARC2_DROME ARC2_DROME] Self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer. Arc2 protein is released from cells in extracellular vesicles that mediate the transfer of mRNA into neighboring cells.[UniProtKB:Q7K1U0]
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== Publication Abstract from PubMed ==
Synaptic plasticity is vital for brain function and memory formation. One of the key proteins in long-term synaptic plasticity and memory is the activity-regulated cytoskeleton-associated protein (Arc). Mammalian Arc forms virus-like capsid structures in a process requiring the N-terminal domain and contains two C-terminal lobes that are structural homologues to retroviral capsids. Drosophila has two isoforms of Arc, dArc1 and dArc2, with low sequence similarity to mammalian Arc, but lacking a large N-terminal domain. Both dArc isoforms are related to the Ty3/gypsy retrotransposon capsid, consisting of N- and C-terminal lobes. Structures of dArc1, as well as capsids formed by both dArc isoforms, have been recently determined. We carried out structural characterization of the four individual dArc lobe domains. As opposed to the corresponding mammalian Arc lobe domains, which are monomeric, the dArc lobes were all oligomeric in solution, indicating a strong propensity for homophilic interactions. A truncated N-lobe from dArc2 formed a domain-swapped dimer in the crystal structure, resulting in a novel dimer interaction that could be relevant for capsid assembly or other dArc functions. This domain-swapped structure resembles the dimeric protein C of flavivirus capsids, as well as the structure of histones dimers, domain-swapped transcription factors, and membrane-interacting BAK domains. The strong oligomerization properties of the isolated dArc lobe domains explain the ability of dArc to form capsids in the absence of any large N-terminal domain, in contrast to the mammalian protein.

Crystal and solution structures reveal oligomerization of individual capsid homology domains of Drosophila Arc.,Hallin EI, Markusson S, Bottger L, Torda AE, Bramham CR, Kursula P PLoS One. 2021 May 14;16(5):e0251459. doi: 10.1371/journal.pone.0251459., eCollection 2021. PMID:33989344<ref>PMID:33989344</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6sib" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Hallin EI]]
[[Category: Kursula P]]