View source for 6sla


==Crystal structure of isomerase PaaG mutant - D136N with Oxepin-CoA==
<StructureSection load='6sla' size='340' side='right'caption='[[6sla]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6sla]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_JL-18 Thermus thermophilus JL-18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SLA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHQ:~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl]+2-(2,5-dihydrooxepin-7-yl)ethanethioate'>LHQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sla OCA], [https://pdbe.org/6sla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sla RCSB], [https://www.ebi.ac.uk/pdbsum/6sla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sla ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H9ZNW0_THETH H9ZNW0_THETH] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Numerous aromatic compounds are aerobically degraded in bacteria via the central intermediate phenylacetic acid (paa). In one of the key steps of this widespread catabolic pathway, 1,2-epoxyphenylacetyl-CoA is converted by PaaG into the heterocyclic oxepin-CoA. PaaG thereby elegantly generates an alpha,beta-unsaturated CoA ester that is predisposed to undergo beta-oxidation subsequent to hydrolytic ring-cleavage. Moreover, oxepin-CoA serves as a precursor for secondary metabolites (e.g., tropodithietic acid) that act as antibiotics and quorum-sensing signals. Here we verify that PaaG adopts a second role in aromatic catabolism by converting cis-3,4-didehydroadipoyl-CoA into trans-2,3-didehydroadipoyl-CoA and corroborate a Delta(3),Delta(2)-enoyl-CoA isomerase-like proton shuttling mechanism for both distinct substrates. Biochemical and structural investigations of PaaG reveal active site adaptations to the structurally different substrates and provide detailed insight into catalysis and control of stereospecificity. This work elucidates the mechanism of action of unusual isomerase PaaG and sheds new light on the ubiquitous enoyl-CoA isomerases of the crotonase superfamily.

Structural and Mechanistic Basis of an Oxepin-CoA Forming Isomerase in Bacterial Primary and Secondary Metabolism.,Spieker M, Saleem-Batcha R, Teufel R ACS Chem Biol. 2019 Nov 18. doi: 10.1021/acschembio.9b00742. PMID:31689071<ref>PMID:31689071</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6sla" style="background-color:#fffaf0;"></div>

==See Also==
*[[Enoyl-CoA hydratase 3D structures|Enoyl-CoA hydratase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus JL-18]]
[[Category: Saleem-Batcha R]]
[[Category: Spieker M]]
[[Category: Teufel R]]