View source for 6sls


==Flavin-dependent tryptophan 6-halogenase Thal in complex with FAD==
<StructureSection load='6sls' size='340' side='right'caption='[[6sls]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6sls]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_albogriseolus Streptomyces albogriseolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SLS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sls OCA], [https://pdbe.org/6sls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sls RCSB], [https://www.ebi.ac.uk/pdbsum/6sls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sls ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A1E280_STRAO A1E280_STRAO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flavin-dependent halogenases require FADH2 , O2 and halide salts to halogenate their substrates. We describe crystal structures of the tryptophan 6-halogenase Thal in complex with FAD or with both tryptophan and FAD. If tryptophan and FAD were soaked simultaneously both ligands showed impaired binding and in some cases only the AMP or the adenosine moiety of FAD was resolved, suggesting that tryptophan binding mainly increases mobility of the FMN moiety. This confirms a negative cooperativity between substrate and cofactor binding previously described for other tryptophan halogenases. Binding of substrate to tryptophan halogenases reduces the affinity for the oxidized cofactor FAD presumably to facilitate regeneration of FADH2 by flavin reductases. This article is protected by copyright. All rights reserved.

Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.,Moritzer AC, Niemann HH Protein Sci. 2019 Oct 7. doi: 10.1002/pro.3739. PMID:31589794<ref>PMID:31589794</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6sls" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces albogriseolus]]
[[Category: Moritzer A]]
[[Category: Niemann HH]]