View source for 6smz


==Crystal structure of SLA Reductase YihU from E. Coli in complex with NADH==
<StructureSection load='6smz' size='340' side='right'caption='[[6smz]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6smz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SMZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6smz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smz OCA], [https://pdbe.org/6smz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6smz RCSB], [https://www.ebi.ac.uk/pdbsum/6smz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6smz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SQUU_ECOLI SQUU_ECOLI] Reduces 3-sulfolactaldehyde (SLA) to 2,3-dihydroxypropane 1-sulfonate (DHPS) (PubMed:24463506, Ref.6). Metabolite profiling studies showed that the enzyme also catalyzes in vitro the NADH-dependent reduction of succinic semialdehyde (SSA) to 4-hydroxybutyrate (GHB), and that it could be involved in the metabolism of SSA, and other potentially toxic intermediates that may accumulate under stress conditions (PubMed:19372223). However, the enzyme exhibits a 42,000-fold greater catalytic efficiency for the reduction of SLA over SSA (Ref.6). Shows no detectable activity on the analogous glycolytic intermediate glyceraldehyde-3-phosphate (Ref.6).<ref>PMID:19372223</ref> <ref>PMID:24463506</ref> <ref>PMID:24463506</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Davies GJ]]
[[Category: Sharma M]]