View source for 6su8


==Highly thermostable endoglucanase Cel7B==
<StructureSection load='6su8' size='340' side='right'caption='[[6su8]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6su8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rasamsonia_emersonii Rasamsonia emersonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SU8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6su8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6su8 OCA], [https://pdbe.org/6su8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6su8 RCSB], [https://www.ebi.ac.uk/pdbsum/6su8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6su8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/W8P1L2_TALEM W8P1L2_TALEM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose. Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge. While substantial biochemical and structural data is available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far. Here we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B. The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate binding module (CBM). With a temperature optimum of 80 degrees C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B. We improved both binding and kinetics in a chimeric variant of ReCel7B and a CBM, while we observe the opposite effect when the CBM was removed in TrCel7B. The crystal structure of ReCel7B was determined at 2.48 A resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively. Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate binding cleft. An increased number of glycosylations were identified in ReCel7B and we propose a stabilizing mechanism for one of the glycans. Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding and cellulose accessibility in GH7 endoglucanases.

Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.,Schiano-di-Cola C, Kolaczkowski B, Sorensen TH, Christensen SJ, Cavaleiro AM, Windahl MS, Borch K, Morth JP, Westh P FEBS J. 2019 Nov 21. doi: 10.1111/febs.15151. PMID:31755197<ref>PMID:31755197</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6su8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rasamsonia emersonii]]
[[Category: Borch K]]
[[Category: Morth JP]]
[[Category: Schiano-di-Cola C]]
[[Category: Westh P]]