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==NMR structure of KRAS32R G9T conformer G-quadruplex within KRAS promoter region==
<StructureSection load='6suu' size='340' side='right'caption='[[6suu]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6suu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SUU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6suu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6suu OCA], [https://pdbe.org/6suu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6suu RCSB], [https://www.ebi.ac.uk/pdbsum/6suu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6suu ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
KRAS is one of the most mutated oncogenes and still considered an undruggable target. An alternative strategy would consist in targeting its gene rather than the protein, specifically the formation of G-quadruplexes (G4) in its promoter. G4 are secondary structures implicated in biological processes, which can be formed among G-rich DNA (or RNA) sequences. Here we have studied the major conformations of the commonly known KRAS 32R, or simply 32R, a 32 residue sequence within the KRAS Nuclease Hypersensitive Element (NHE) region. We have determined the structure of the two major stable conformers that 32R can adopt and which display slow equilibrium (&gt;ms) with each other. By using different biophysical methods, we found that the nucleotides G9, G25, G28 and G32 are particularly implicated in the exchange between these two conformations. We also showed that a triad at the 3' end further stabilizes one of the G4 conformations, while the second conformer remains more flexible and less stable.

Structure of two G-quadruplexes in equilibrium in the KRAS promoter.,Marquevielle J, Robert C, Lagrabette O, Wahid M, Bourdoncle A, Xodo LE, Mergny JL, Salgado GF Nucleic Acids Res. 2020 May 20. pii: 5841130. doi: 10.1093/nar/gkaa387. PMID:32432667<ref>PMID:32432667</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Marquevielle J]]
[[Category: Salgado G]]