View source for 6t69


==Crystal structure of Toxoplasma gondii Morn1(V shape)==
<StructureSection load='6t69' size='340' side='right'caption='[[6t69]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6t69]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_VEG Toxoplasma gondii VEG]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T69 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t69 OCA], [https://pdbe.org/6t69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t69 RCSB], [https://www.ebi.ac.uk/pdbsum/6t69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t69 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0F7VBC6_TOXGV A0A0F7VBC6_TOXGV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes and eukaryotes. Despite this ubiquity, they remain poorly characterised at both a structural and a functional level compared to other common repeats. In functional terms, they are often assumed to be lipid-binding modules that mediate membrane targeting. We addressed this putative activity by focusing on a protein composed solely of MORN repeats-Trypanosoma brucei MORN1. Surprisingly, no evidence for binding to membranes or lipid vesicles by TbMORN1 could be obtained either in vivo or in vitro. Conversely, TbMORN1 did interact with individual phospholipids. High- and low-resolution structures of the MORN1 protein from Trypanosoma brucei and homologous proteins from the parasites Toxoplasma gondii and Plasmodium falciparum were obtained using a combination of macromolecular crystallography, small-angle X-ray scattering, and electron microscopy. This enabled a first structure-based definition of the MORN repeat itself. Furthermore, all three structures dimerised via their C-termini in an antiparallel configuration. The dimers could form extended or V-shaped quaternary structures depending on the presence of specific interface residues. This work provides a new perspective on MORN repeats, showing that they are protein-protein interaction modules capable of mediating both dimerisation and oligomerisation.

Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.,Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trubestein L, Niedermuller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K PLoS One. 2020 Dec 9;15(12):e0242677. doi: 10.1371/journal.pone.0242677., eCollection 2020. PMID:33296386<ref>PMID:33296386</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6t69" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Toxoplasma gondii VEG]]
[[Category: Djinovic-Carugo K]]
[[Category: Grishkovskaya I]]
[[Category: Kostan J]]
[[Category: Morriswood B]]
[[Category: Sajko S]]