View source for 6t72


==Structure of the RsaA N-terminal domain bound to LPS==
<SX load='6t72' size='340' side='right' viewer='molstar' caption='[[6t72]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6t72]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T72 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MRH:4-acetamido-4,6-dideoxy-alpha-D-mannopyranose'>MRH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t72 OCA], [https://pdbe.org/6t72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t72 RCSB], [https://www.ebi.ac.uk/pdbsum/6t72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t72 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SLAP_CAUVC SLAP_CAUVC] The S-layer is a paracrystalline mono-layered assembly of proteins which coats the surface of bacteria. Probably acts as a physical barrier to parasites and lytic enzymes.
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== Publication Abstract from PubMed ==
Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. Using native mass spectrometry and molecular dynamics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide binding and S-layer assembly is regulated by calcium. Finally, we present a near-atomic resolution in situ structure of the complete S-layer using cellular electron cryotomography, showing S-layer arrangement at the tip of the O-antigen. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.

In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer.,von Kugelgen A, Tang H, Hardy GG, Kureisaite-Ciziene D, Brun YV, Stansfeld PJ, Robinson CV, Bharat TAM Cell. 2020 Jan 23;180(2):348-358.e15. doi: 10.1016/j.cell.2019.12.006. Epub 2019 , Dec 26. PMID:31883796<ref>PMID:31883796</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6t72" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Caulobacter vibrioides CB15]]
[[Category: Large Structures]]
[[Category: Bharat TAM]]
[[Category: Von Kuegelgen A]]