View source for 6tkt


==Structure of the bacterial toxin phenomycin==
<StructureSection load='6tkt' size='340' side='right'caption='[[6tkt]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6tkt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_roseoverticillatus Streptomyces roseoverticillatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TKT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tkt OCA], [https://pdbe.org/6tkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tkt RCSB], [https://www.ebi.ac.uk/pdbsum/6tkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tkt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q53805_9ACTN Q53805_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phenomycin is a bacterial mini-protein of 89 amino acids discovered more than 50 years ago with toxicity in the nanomolar regime toward mammalian cells. The protein inhibits the function of the eukaryotic ribosome in cell-free systems and appears to target translation initiation. Several fundamental questions concerning the cellular activity of phenomycin, however, have remained unanswered. In this paper, we have used morphological profiling to show that direct inhibition of translation underlies the toxicity of phenomycin in cells. We have performed studies of the cellular uptake mechanism of phenomycin, showing that endosomal escape is the toxicity-limiting step, and we have solved a solution phase high-resolution structure of the protein using NMR spectroscopy. Through bioinformatic as well as functional comparisons between phenomycin and two homologs, we have identified a peptide segment, which constitutes one of two loops in the structure that is critical for the toxicity of phenomycin.

Structure and Function of the Bacterial Protein Toxin Phenomycin.,Hansen BK, Larsen CK, Nielsen JT, Svenningsen EB, Van LB, Jacobsen KM, Bjerring M, Flygaard RK, Jenner LB, Nejsum LN, Brodersen DE, Mulder FAA, Torring T, Poulsen TB Structure. 2020 Mar 24. pii: S0969-2126(20)30075-7. doi:, 10.1016/j.str.2020.03.003. PMID:32220302<ref>PMID:32220302</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tkt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces roseoverticillatus]]
[[Category: Mulder FAA]]
[[Category: Nielsen JT]]
[[Category: Poulsen T]]
[[Category: Toerring T]]