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==Crystal structure of the unconventional kinetochore protein Perkinsela sp. KKT2a central domain==
<StructureSection load='6tlx' size='340' side='right'caption='[[6tlx]], [[Resolution|resolution]] 2.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6tlx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Perkinsela_sp._ccap_1560/4 Perkinsela sp. ccap 1560/4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TLX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6tly|6tly]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XU18_4017 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314962 Perkinsela sp. CCAP 1560/4])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlx OCA], [https://pdbe.org/6tlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tlx RCSB], [https://www.ebi.ac.uk/pdbsum/6tlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlx ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The kinetochore is the macromolecular protein complex that assembles onto centromeric DNA and binds spindle microtubules. Evolutionarily divergent kinetoplastids have an unconventional set of kinetochore proteins. It remains unknown how kinetochores assemble at centromeres in these organisms. Here, we characterize KKT2 and KKT3 in the kinetoplastid parasite Trypanosoma brucei. In addition to the N-terminal kinase domain and C-terminal divergent polo boxes, these proteins have a central domain of unknown function. We show that KKT2 and KKT3 are important for the localization of several kinetochore proteins and that their central domains are sufficient for centromere localization. Crystal structures of the KKT2 central domain from two divergent kinetoplastids reveal a unique zinc-binding domain (termed the CL domain for centromere localization), which promotes its kinetochore localization in T. brucei. Mutations in the equivalent domain in KKT3 abolish its kinetochore localization and function. Our work shows that the unique central domains play a critical role in mediating the centromere localization of KKT2 and KKT3.

Kinetoplastid kinetochore proteins KKT2 and KKT3 have unique centromere localization domains.,Marciano G, Ishii M, Nerusheva OO, Akiyoshi B J Cell Biol. 2021 Aug 2;220(8). pii: 212224. doi: 10.1083/jcb.202101022. Epub, 2021 Jun 3. PMID:34081090<ref>PMID:34081090</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tlx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Perkinsela sp. ccap 1560/4]]
[[Category: Akiyoshi, B]]
[[Category: Ishii, M]]
[[Category: Marciano, G]]
[[Category: Nerusheva, O]]
[[Category: Cell cycle]]
[[Category: Kinetochore]]
[[Category: Kinetoplastid]]
[[Category: Kkt2]]
[[Category: Zinc finger]]