View source for 6ts9


==Crystal structure of GES-5 carbapenemase==
<StructureSection load='6ts9' size='340' side='right'caption='[[6ts9]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ts9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TS9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ts9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ts9 OCA], [https://pdbe.org/6ts9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ts9 RCSB], [https://www.ebi.ac.uk/pdbsum/6ts9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ts9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BLAG5_KLEPN BLAG5_KLEPN] Confers resistance to penicillins, cephalosporins and carbapenems (PubMed:20696873, PubMed:27590339). Has carbapenem-hydrolyzing activity (PubMed:27590339).<ref>PMID:20696873</ref> <ref>PMID:27590339</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The worldwide spread of beta-lactamases able to hydrolyze last resort carbapenems contributes to the antibiotic resistance problem and menaces the successful antimicrobial treatment of clinically relevant pathogens. Class A carbapenemases include members of the KPC and GES families. While drugs against KPC-type carbapenemases have recently been approved, for GES-type enzymes, no inhibitors have yet been introduced in therapy. Thus, GES carbapenemases represent important drug targets. Here, we present an in silico screening against the most prevalent GES carbapenemase, GES-5, using a lead-like compound library of commercially available compounds. The most promising candidates were selected for in vitro validation in biochemical assays against recombinant GES-5 leading to four derivatives active as high micromolar competitive inhibitors. For the best inhibitors, the ability to inhibit KPC-2 was also evaluated. The discovered inhibitors constitute promising starting points for hit to lead optimization.

Targeting the Class A Carbapenemase GES-5 via Virtual Screening.,Klein R, Cendron L, Montanari M, Bellio P, Celenza G, Maso L, Tondi D, Brenk R Biomolecules. 2020 Feb 14;10(2). pii: biom10020304. doi: 10.3390/biom10020304. PMID:32075131<ref>PMID:32075131</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ts9" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Bellio C]]
[[Category: Brenk R]]
[[Category: Celenza G]]
[[Category: Cendron L]]
[[Category: Klein R]]
[[Category: Maso L]]
[[Category: Montanari M]]
[[Category: Tondi D]]