View source for 6tsw


==Isometric capsid of empty GTA particle computed with I4(I,n25r) symmetry==
<StructureSection load='6tsw' size='340' side='right'caption='[[6tsw]], [[Resolution|resolution]] 4.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6tsw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus_DE442 Rhodobacter capsulatus DE442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TSW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.03&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tsw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tsw OCA], [https://pdbe.org/6tsw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tsw RCSB], [https://www.ebi.ac.uk/pdbsum/6tsw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tsw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D5ATZ3_RHOCB D5ATZ3_RHOCB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.

Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663<ref>PMID:32541663</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tsw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodobacter capsulatus DE442]]
[[Category: Bardy P]]
[[Category: Beatty JT]]
[[Category: Fuzik T]]
[[Category: Hrebik D]]
[[Category: Pantucek R]]
[[Category: Plevka P]]