View source for 6u75


==Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ2==
<StructureSection load='6u75' size='340' side='right'caption='[[6u75]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6u75]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U75 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u75 OCA], [https://pdbe.org/6u75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u75 RCSB], [https://www.ebi.ac.uk/pdbsum/6u75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u75 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SIZ2_YEAST SIZ2_YEAST] May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.<ref>PMID:11333221</ref> <ref>PMID:12761287</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Repair of DNA double-stranded breaks by homologous recombination (HR) is dependent on DNA end resection and on post-translational modification of repair factors. In budding yeast, single-stranded DNA is coated by replication protein A (RPA) following DNA end resection, and DNA-RPA complexes are then SUMO-modified by the E3 ligase Siz2 to promote repair. Here, we show using enzymatic assays that DNA duplexes containing 3' single-stranded DNA overhangs increase the rate of RPA SUMO modification by Siz2. The SAP domain of Siz2 binds DNA duplexes and makes a key contribution to this process as highlighted by models and a crystal structure of Siz2 and by assays performed using protein mutants. Enzymatic assays performed using DNA that can accommodate multiple RPA proteins suggest a model in which the SUMO-RPA signal is amplified by successive rounds of Siz2-dependent SUMO modification of RPA and dissociation of SUMO-RPA at the junction between single- and double-stranded DNA. Our results provide insights on how DNA architecture scaffolds a substrate and E3 ligase to promote SUMO modification in the context of DNA repair.

DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.,Cappadocia L, Kochanczyk T, Lima CD EMBO J. 2021 Nov 15;40(22):e103787. doi: 10.15252/embj.2019103787. Epub 2021 Sep , 29. PMID:34585421<ref>PMID:34585421</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6u75" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Cappadocia L]]
[[Category: Lima CD]]