View source for 6uio


==Crystal structure of mouse CRES (Cystatin-Related Epididymal Spermatogenic)==
<StructureSection load='6uio' size='340' side='right'caption='[[6uio]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6uio]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UIO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uio OCA], [https://pdbe.org/6uio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uio RCSB], [https://www.ebi.ac.uk/pdbsum/6uio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uio ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CST8_MOUSE CST8_MOUSE] Performs a specialized role during sperm development and maturation.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The epididymal lumen contains a complex cystatin-rich nonpathological amyloid matrix with putative roles in sperm maturation and sperm protection. Given our growing understanding for the biological function of this and other functional amyloids, the problem still remains: how functional amyloids assemble including their initial transition to early oligomeric forms. To examine this, we developed a protocol for the purification of nondenatured mouse CRES, a component of the epididymal amyloid matrix, allowing us to examine its assembly to amyloid under conditions that may mimic those in vivo. Herein we use X-ray crystallography, solution-state NMR, and solid-state NMR to follow at the atomic level the assembly of the CRES amyloidogenic precursor as it progressed from monomeric folded protein to an advanced amyloid. We show the CRES monomer has a typical cystatin fold that assembles into highly branched amyloid matrices, comparable to those in vivo, by forming beta-sheet assemblies that our data suggest occur via two distinct mechanisms: a unique conformational switch of a highly flexible disulfide-anchored loop to a rigid beta-strand and by traditional cystatin domain swapping. Our results provide key insight into our understanding of functional amyloid assembly by revealing the earliest structural transitions from monomer to oligomer and by showing that some functional amyloid structures may be built by multiple and distinctive assembly mechanisms.

Maturation of the functional mouse CRES amyloid from globular form.,Hewetson A, Khan NH, Dominguez MJ, Do HQ, Kusko RE, Borcik CG, Rigden DJ, Keegan RM, Sutton RB, Latham MP, Wylie BJ, Cornwall GA Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16363-16372. doi:, 10.1073/pnas.2006887117. Epub 2020 Jun 29. PMID:32601205<ref>PMID:32601205</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6uio" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Cornwall GA]]
[[Category: Dominguez MJ]]
[[Category: Hewetson A]]
[[Category: Sutton RB]]