View source for 6umr


==Structure of DUF89 - D291A mutant==
<StructureSection load='6umr' size='340' side='right'caption='[[6umr]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6umr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UMR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6umr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6umr OCA], [https://pdbe.org/6umr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6umr RCSB], [https://www.ebi.ac.uk/pdbsum/6umr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6umr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARMT1_HUMAN ARMT1_HUMAN] Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate (By similarity). Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism (By similarity). Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues (PubMed:25732820). Possibly methylates PCNA, suggesting it is involved in the DNA damage response (PubMed:25732820).[UniProtKB:Q04371]<ref>PMID:25732820</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metabolite damage control is a critical but poorly defined aspect of cellular biochemistry, which likely involves many of the so far functionally uncharacterized protein domain (domains of unknown function; DUFs). We have determined the crystal structure of the human DUF89 protein product of the C6ORF211 gene to 1.85 A. The crystal structure shows that the protein contains a core alpha-beta-alpha fold with an active site-bound metal ion and alpha-helical bundle N-terminal cap, which are both conserved features of subfamily III DUF89 domains. The biochemical activities of the human protein are conserved with those of a previously characterized budding yeast homolog, where an in vitro phosphatase activity is supported by divalent cations that include Co(2+), Ni(2+), Mn(2+) or Mg(2+). Full steady-state kinetics parameters of human DUF89 using a standard PNPP phosphatase assay revealed a six times higher catalytic efficiency in presence of Co(2+) compared to Mg(2+). The human enzyme targets a number of phosphate substrates similar to the budding yeast homolog, while it lacks a previously indicated methyltransferase activity. The highest activity on substrate was observed with fructose-1-phosphate, a potent glycating agent, and thus human DUF89 phosphatase activity may also play a role in limiting the buildup of phospho-glycan species and their related damaged metabolites.

Human ARMT1 structure and substrate specificity indicates that it is a DUF89 family damage-control phosphatase.,Dennis TN, Kenjic N, Kang AS, Lowenson JD, Kirkwood JS, Clarke SG, Jefferson P Perry J J Struct Biol. 2020 Jul 15:107576. doi: 10.1016/j.jsb.2020.107576. PMID:32682077<ref>PMID:32682077</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6umr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Dennis TN]]
[[Category: Kenjic N]]
[[Category: Perry JJ]]