View source for 6upa


==Crystal Structure of GTPase Domain of Human Septin 2/Septin 6 Heterocomplex==
<StructureSection load='6upa' size='340' side='right'caption='[[6upa]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6upa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UPA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6upa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upa OCA], [https://pdbe.org/6upa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6upa RCSB], [https://www.ebi.ac.uk/pdbsum/6upa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6upa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEPT6_HUMAN SEPT6_HUMAN] Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.<ref>PMID:17803907</ref> <ref>PMID:17229681</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The assembly of a septin filament requires that homologous monomers must distinguish between one another in establishing appropriate interfaces with their neighbors. To understand this phenomenon at the molecular level, we present the first four crystal structures of heterodimeric septin complexes. We describe in detail the two distinct types of G-interface present within the octameric particles, which must polymerize to form filaments. These are formed between SEPT2 and SEPT6 and between SEPT7 and SEPT3, and their description permits an understanding of the structural basis for the selectivity necessary for correct filament assembly. By replacing SEPT6 by SEPT8 or SEPT11, it is possible to rationalize Kinoshita'spostulate, which predicts the exchangeability of septins from within a subgroup. Switches I and II, which in classical small GTPases provide a mechanism for nucleotide-dependent conformational change, have been repurposed in septins to play a fundamental role in molecular recognition. Specifically, it is switch I which holds the key to discriminating between the two different G-interfaces. Moreover, residues which are characteristic for a given subgroup play subtle, but pivotal, roles in guaranteeing that the correct interfaces are formed.

Molecular Recognition at Septin Interfaces: The Switches Hold the Key.,Rosa HVD, Leonardo DA, Brognara G, Brandao-Neto J, D'Muniz Pereira H, Araujo APU, Garratt RC J Mol Biol. 2020 Sep 7. pii: S0022-2836(20)30531-3. doi:, 10.1016/j.jmb.2020.09.001. PMID:32910969<ref>PMID:32910969</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6upa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Araujo APU]]
[[Category: Brandao-Neto J]]
[[Category: Garratt RC]]
[[Category: Martins C]]
[[Category: Pereira HM]]
[[Category: Rosa HVD]]