View source for 6usc


==Structure of Human Intelectin-1 in complex with KO==
<StructureSection load='6usc' size='340' side='right'caption='[[6usc]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6usc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6USC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6USC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KO2:PROP-2-EN-1-YL+D-GLYCERO-ALPHA-D-TALO-OCT-2-ULOPYRANOSIDONIC+ACID'>KO2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6usc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6usc OCA], [https://pdbe.org/6usc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6usc RCSB], [https://www.ebi.ac.uk/pdbsum/6usc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6usc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ITLN1_HUMAN ITLN1_HUMAN] Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism.<ref>PMID:11313366</ref> <ref>PMID:16531507</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recognition of distinct glycans is central to biology, and lectins mediate this function. Lectin glycan preferences are usually centered on specific monosaccharides. In contrast, human intelectin-1 (hItln-1, also known as Omentin-1) is a soluble lectin that binds a range of microbial sugars, including beta-D-galactofuranose (beta-Galf), D-glycerol 1-phosphate, D-glycero-D-talo-oct-2-ulosonic acid (KO), and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Though these saccharides differ dramatically in structure, they share a common feature-an exocyclic vicinal diol. How and whether such a small fragment is sufficient for recognition was unclear. We tested several glycans with this epitope and found that L-glycero-alpha-D-manno-heptose and D-glycero-alpha-D-manno-heptose possess the critical diol motif yet bind weakly. To better understand hItln-1 recognition, we determined the structure of the hItln-1.KO complex using X-ray crystallography, and our 1.59-A resolution structure enabled unambiguous assignment of the bound KO conformation. This carbohydrate conformation was present in &gt;97% of the KDO/KO structures in the Protein Data Bank. Bioinformatic analysis revealed that KO and KDO adopt a common conformation, while heptoses prefer different conformers. The preferred conformers of KO and KDO favor hItln-1 engagement, but those of the heptoses do not. Natural bond orbital (NBO) calculations suggest these observed conformations, including the side chain orientations, are stabilized by not only steric but also stereoelectronic effects. Thus, our data highlight a role for stereoelectronic effects in dictating the specificity of glycan recognition by proteins. Finally, our finding that hItln-1 avoids binding prevalent glycans with a terminal 1,2 diol (e.g., NeuAc, and L-glycero-alpha-D-manno-heptose) suggests the lectin has evolved to recognize distinct bacterial species.

Stereoelectronic effects impact glycan recognition.,McMahon CM, Isabella CR, Windsor IW, Kosma P, Raines RT, Kiessling LL J Am Chem Soc. 2020 Jan 13. doi: 10.1021/jacs.9b11699. PMID:31930911<ref>PMID:31930911</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6usc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Isabella CR]]
[[Category: Kiessling LL]]
[[Category: Kosma P]]
[[Category: Raines RT]]
[[Category: Windsor IW]]