View source for 6v86


==Parainfluenza virus 5 L-P complex with an alternate conformation of the CD-MTase-CTD module==
<SX load='6v86' size='340' side='right' viewer='molstar' caption='[[6v86]], [[Resolution|resolution]] 4.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6v86]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_virus_5_(strain_W3) Simian virus 5 (strain W3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V86 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.63&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v86 OCA], [https://pdbe.org/6v86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v86 RCSB], [https://www.ebi.ac.uk/pdbsum/6v86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v86 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/L_PIV5 L_PIV5] RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.[UniProtKB:P03523]  RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.[UniProtKB:P03523]

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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[[Category: Large Structures]]
[[Category: Abdella R]]
[[Category: He Y]]