View source for 6vfi


==De novo designed octahedral nanoparticle O43_dn18==
<StructureSection load='6vfi' size='340' side='right'caption='[[6vfi]], [[Resolution|resolution]] 4.54&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VFI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.54&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vfi OCA], [https://pdbe.org/6vfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vfi RCSB], [https://www.ebi.ac.uk/pdbsum/6vfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vfi ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Multivalent presentation of viral glycoproteins can substantially increase the elicitation of antigen-specific antibodies. To enable a new generation of anti-viral vaccines, we designed self-assembling protein nanoparticles with geometries tailored to present the ectodomains of influenza, HIV, and RSV viral glycoprotein trimers. We first de novo designed trimers tailored for antigen fusion, featuring N-terminal helices positioned to match the C termini of the viral glycoproteins. Trimers that experimentally adopted their designed configurations were incorporated as components of tetrahedral, octahedral, and icosahedral nanoparticles, which were characterized by cryo-electron microscopy and assessed for their ability to present viral glycoproteins. Electron microscopy and antibody binding experiments demonstrated that the designed nanoparticles presented antigenically intact prefusion HIV-1 Env, influenza hemagglutinin, and RSV F trimers in the predicted geometries. This work demonstrates that antigen-displaying protein nanoparticles can be designed from scratch, and provides a systematic way to investigate the influence of antigen presentation geometry on the immune response to vaccination.

Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens.,Ueda G, Antanasijevic A, Fallas JA, Sheffler W, Copps J, Ellis D, Hutchinson GB, Moyer A, Yasmeen A, Tsybovsky Y, Park YJ, Bick MJ, Sankaran B, Gillespie RA, Brouwer PJ, Zwart PH, Veesler D, Kanekiyo M, Graham BS, Sanders RW, Moore JP, Klasse PJ, Ward AB, King NP, Baker D Elife. 2020 Aug 4;9. pii: 57659. doi: 10.7554/eLife.57659. PMID:32748788<ref>PMID:32748788</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Antanasijevic A]]
[[Category: Ward AB]]