View source for 6vgs


==Alpha-ketoisovalerate decarboxylase (KivD) from Lactococcus lactis, thermostable mutant==
<StructureSection load='6vgs' size='340' side='right'caption='[[6vgs]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vgs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VGS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vgs OCA], [https://pdbe.org/6vgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vgs RCSB], [https://www.ebi.ac.uk/pdbsum/6vgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vgs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0B8QZ66_LACLL A0A0B8QZ66_LACLL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cost competitive conversion of biomass-derived sugars into biofuel will require high yields, high volumetric productivities and high titers. Suitable production parameters are hard to achieve in cell-based systems because of the need to maintain life processes. As a result, next-generation biofuel production in engineered microbes has yet to match the stringent cost targets set by petroleum fuels. Removing the constraints imposed by having to maintain cell viability might facilitate improved production metrics. Here, we report a cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity of 4 g L(-1) h(-1), a titer of 275 g L(-1) and 95% yield over the course of nearly 5 days. These production metrics exceed even the highly developed ethanol fermentation process. Our results suggest that moving beyond cells has the potential to expand what is possible for bio-based chemical production.

Isobutanol production freed from biological limits using synthetic biochemistry.,Sherkhanov S, Korman TP, Chan S, Faham S, Liu H, Sawaya MR, Hsu WT, Vikram E, Cheng T, Bowie JU Nat Commun. 2020 Aug 27;11(1):4292. doi: 10.1038/s41467-020-18124-1. PMID:32855421<ref>PMID:32855421</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vgs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lactococcus lactis subsp. lactis]]
[[Category: Large Structures]]
[[Category: Bowie JU]]
[[Category: Chan S]]
[[Category: Korman TP]]
[[Category: Sawaya MR]]