View source for 6vjv


==Crystal structure of the Prochlorococcus phage (myovirus P-SSM2) ferredoxin at 1.6 Angstroms==
<StructureSection load='6vjv' size='340' side='right'caption='[[6vjv]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vjv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Prochlorococcus_phage_P-SSM2 Prochlorococcus phage P-SSM2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VJV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjv OCA], [https://pdbe.org/6vjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vjv RCSB], [https://www.ebi.ac.uk/pdbsum/6vjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q58M74_BPPRM Q58M74_BPPRM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Marine cyanobacteria are infected by phages whose genomes encode ferredoxin (Fd) electron carriers. These Fds are thought to redirect the energy harvested from light to phage-encoded oxidoreductases that enhance viral fitness, but it is unclear how the biophysical properties and partner specificities of phage Fds relate to those in photosynthetic organisms. Here, results of a bioinformatics analysis using a sequence similarity network revealed that phage Fds are most closely related to cyanobacterial Fds that transfer electrons from photosystems to oxidoreductases involved in nutrient assimilation. Structural analysis of myovirus P-SSM2 Fd (pssm2-Fd), which infects the cyanobacterium Prochlorococcus marinus, revealed a high similarity to cyanobacterial Fds (&lt;/= 0.5 A root-mean-square deviation). Additionally, pssm2-Fd exhibited a low midpoint reduction potential (-336 mV versus standard hydrogen electrode) similar to other photosynthetic Fds, albeit had lower thermostability (Tm = 28 degrees C) than many other Fds. When expressed in an Escherichia coli strain deficient in sulfite assimilation, pssm2-Fd complemented bacterial growth when co-expressed with a P. marinus sulfite reductase, revealing that pssm2-Fd can transfer electrons to a host protein involved in nutrient assimilation. The high structural similarity with cyanobacterial Fds and reactivity with a host sulfite reductase suggest that phage Fds evolved to transfer electrons to cyanobacterial-encoded oxidoreductases.

Prochlorococcus phage ferredoxin: Structural characterization and electron transfer to cyanobacterial sulfite reductases.,Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 10.1074/jbc.RA120.013501. PMID:32434930<ref>PMID:32434930</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vjv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Prochlorococcus phage P-SSM2]]
[[Category: Atkinson JT]]
[[Category: Bennett GN]]
[[Category: Campbell IJ]]
[[Category: Kahanda D]]
[[Category: Miller MD]]
[[Category: Olmos Jr JL]]
[[Category: Phillips Jr GN]]
[[Category: Silberg JJ]]
[[Category: Sparks N]]
[[Category: Xu W]]