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==Solution NMR structure of the myristoylated feline immunodeficiency virus matrix protein==
<StructureSection load='6wa3' size='340' side='right'caption='[[6wa3]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WA3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wa3 OCA], [https://pdbe.org/6wa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wa3 RCSB], [https://www.ebi.ac.uk/pdbsum/6wa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wa3 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
All retroviruses encode a Gag polyprotein containing an N-terminal matrix domain (MA) that anchors Gag to the plasma membrane and recruits envelope glycoproteins to virus assembly sites. Membrane binding by the Gag protein of HIV-1 and most other lentiviruses is dependent on N-terminal myristoylation of MA by host N-myristoyltransferase enzymes (NMTs), which recognize a six-residue "myristoylation signal" with consensus sequence: M1GXXX[ST]. For unknown reasons, the feline immunodeficiency virus (FIV), which infects both domestic and wild cats, encodes a non-consensus myristoylation sequence not utilized by its host or by other mammals (most commonly: M1GNGQG). To explore the evolutionary basis for this sequence, we compared the structure, dynamics, and myristoylation properties of native FIV MA with a mutant protein containing a consensus feline myristoylation motif (MA(NOS)) and examined the impact of MA mutations on virus assembly and ability to support spreading infection. Unexpectedly, myristoylation efficiency of MA(NOS) in Escherichia coli by co-expressed mammalian NMT was reduced by ~70% compared to the wild-type protein. NMR studies revealed that residues of the N-terminal myristoylation signal are fully exposed and mobile in the native protein but partially sequestered in the MA(NOS) chimera, suggesting that the unusual FIV sequence is conserved to promote exposure and efficient myristoylation of the MA N terminus. In contrast, virus assembly studies indicate that the MA(NOS) mutation does not affect virus assembly, but does prevent virus spread, in feline kidney cells. Our findings indicate that residues of the FIV myristoylation sequence play roles in replication beyond NMT recognition and Gag-membrane binding.

Structural and Mechanistic Studies of the Rare Myristoylation Signal of the Feline Immunodeficiency Virus.,Brown JB, Summers HR, Brown LA, Marchant J, Canova PN, O'Hern CT, Abbott ST, Nyaunu C, Maxwell S, Johnson T, Moser MB, Ablan SD, Carter H, Freed EO, Summers MF J Mol Biol. 2020 Jun 26;432(14):4076-4091. doi: 10.1016/j.jmb.2020.05.008. Epub, 2020 May 19. PMID:32442659<ref>PMID:32442659</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6wa3" style="background-color:#fffaf0;"></div>

==See Also==
*[[Gag polyprotein 3D structures|Gag polyprotein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Abbott S]]
[[Category: Ablan SD]]
[[Category: Brown JB]]
[[Category: Brown LA]]
[[Category: Canova PN]]
[[Category: Carter H]]
[[Category: Freed EO]]
[[Category: Johnson T]]
[[Category: Marchant J]]
[[Category: Maxwell S]]
[[Category: Moser M]]
[[Category: Nyaunu C]]
[[Category: O'Hern CT]]
[[Category: Summers HR]]
[[Category: Summers MF]]