View source for 6wbx


==Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S Class 1==
<StructureSection load='6wbx' size='340' side='right'caption='[[6wbx]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6wbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_abcessus"_moore_and_frerichs_1953 "mycobacterium abcessus" moore and frerichs 1953]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WBX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6w98|6w98]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D2E76_26050 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36809 "Mycobacterium abcessus" Moore and Frerichs 1953])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wbx OCA], [https://pdbe.org/6wbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wbx RCSB], [https://www.ebi.ac.uk/pdbsum/6wbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wbx ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium tuberculosis causes tuberculosis, a disease that kills over 1 million people each year. Its cell envelope is a common antibiotic target and has a unique structure due, in part, to two lipidated polysaccharides-arabinogalactan and lipoarabinomannan. Arabinofuranosyltransferase D (AftD) is an essential enzyme involved in assembling these glycolipids. We present the 2.9-A resolution structure of M. abscessus AftD, determined by single-particle cryo-electron microscopy. AftD has a conserved GT-C glycosyltransferase fold and three carbohydrate-binding modules. Glycan array analysis shows that AftD binds complex arabinose glycans. Additionally, AftD is non-covalently complexed with an acyl carrier protein (ACP). 3.4- and 3.5-A structures of a mutant with impaired ACP binding reveal a conformational change, suggesting that ACP may regulate AftD function. Mutagenesis experiments using a conditional knockout constructed in M. smegmatis confirm the essentiality of the putative active site and the ACP binding for AftD function.

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria.,Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F Mol Cell. 2020 May 5. pii: S1097-2765(20)30256-2. doi:, 10.1016/j.molcel.2020.04.014. PMID:32386575<ref>PMID:32386575</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6wbx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Mycobacterium abcessus moore and frerichs 1953]]
[[Category: Large Structures]]
[[Category: Archer, M]]
[[Category: Athayde, D]]
[[Category: Brunton, R]]
[[Category: Carragher, B]]
[[Category: Catalao, M J]]
[[Category: Clarke, O B]]
[[Category: Dandey, V P]]
[[Category: Giacometti, S I]]
[[Category: Kloss, B]]
[[Category: Lowary, T L]]
[[Category: Mancia, F]]
[[Category: Niederweis, M]]
[[Category: Pimentel, M]]
[[Category: Potter, C S]]
[[Category: Raczkowski, A M]]
[[Category: Rodrigues, J]]
[[Category: Rosario, A L]]
[[Category: Tan, Y Z]]
[[Category: Wei, H]]
[[Category: Zhang, L]]
[[Category: Zheng, R B]]
[[Category: Acyl carrier protein]]
[[Category: Glycosyltransferase]]
[[Category: Membrane protein]]
[[Category: Nanodisc]]