View source for 6wjp


==Crystal structure of Arginine Repressor P115Q mutant from the pathogenic bacterium Corynebacterium pseudotuberculosis bound to arginine==
<StructureSection load='6wjp' size='340' side='right'caption='[[6wjp]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6wjp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_pseudotuberculosis_C231 Corynebacterium pseudotuberculosis C231]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WJP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.701&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjp OCA], [https://pdbe.org/6wjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wjp RCSB], [https://www.ebi.ac.uk/pdbsum/6wjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D9QA55_CORP2 D9QA55_CORP2] Regulates arginine biosynthesis genes.[ARBA:ARBA00002095][HAMAP-Rule:MF_00173]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The arginine repressor (ArgR) regulates the expression of genes involved in arginine biosynthesis. Upon attaining a threshold concentration of arginine in the cytoplasm, the trimeric C-terminal domain of ArgR binds three arginines in a shallow surface cleft and subsequently hexamerizes forming a dimer of trimers containing six Arg co-repressor molecules which are buried at the subunit interfaces. The N-terminal domains of this complex bind to the DNA promoter thereby interrupting the transcription of the genes related to Arg biosynthesis. The crystal structures of the wild type and mutant Pro115Gln ArgR from Corynebacterium pseudotuberculosis determined at 1.7 A demonstrate that a single amino acid substitution switches co-repressor specificity from Tyr to Arg. Molecular dynamics simulations indicate that the first step, i.e., the binding of the co-repressor, occurs in the trimeric state and that Pro115Gln ArgR preferentially binds Arg. It was also shown that, in Pro115 ArgR hexamers, the concomitant binding of sodium ions shifts selectivity to Tyr. Structural data combined with phylogenetic analyses of ArgR from C. pseudotuberculosis suggest that substitutions in the binding pocket at position 115 may alter its specificity for amino acids and that the length of the protein interdomain linker can provide further functional flexibility. These results support the existence of alternative ArgR regulatory mechanisms in this pathogenic bacterium.

A single P115Q mutation modulates specificity in the Corynebacterium pseudotuberculosis arginine repressor.,Mariutti RB, Hernandez-Gonzalez JE, Nascimento AFZ, de Morais MAB, Murakami MT, Carareto CMA, Arni RK Biochim Biophys Acta Gen Subj. 2020 Mar 7;1864(7):129597. doi:, 10.1016/j.bbagen.2020.129597. PMID:32156582<ref>PMID:32156582</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6wjp" style="background-color:#fffaf0;"></div>

==See Also==
*[[Arginine repressor 3D structures|Arginine repressor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Corynebacterium pseudotuberculosis C231]]
[[Category: Large Structures]]
[[Category: Arni RK]]
[[Category: Carareto CMA]]
[[Category: Hernandez-Gonzalez JE]]
[[Category: Mariutti RB]]
[[Category: Murakami MT]]
[[Category: Nascimento AFZ]]
[[Category: De Morais MAB]]