View source for 6wqx


==Human PRPK-TPRKB complex==
<StructureSection load='6wqx' size='340' side='right'caption='[[6wqx]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6wqx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WQX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqx OCA], [https://pdbe.org/6wqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wqx RCSB], [https://www.ebi.ac.uk/pdbsum/6wqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TPRKB_HUMAN TPRKB_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mutations of the p53-related protein kinase (PRPK) and TP53RK-binding protein (TPRKB) cause Galloway-Mowat syndrome (GAMOS) and are found in various human cancers. We have previously shown that small compounds targeting PRPK showed anti-cancer activity against colon and skin cancer. Here we present the 2.53 A crystal structure of the human PRPK-TPRKB-AMPPNP (adenylyl-imidodiphosphate) complex. The structure reveals details in PRPK-AMPPNP coordination and PRPK-TPRKB interaction. PRPK appears in an active conformation, albeit lacking the conventional kinase activation loop. We constructed a structural model of the human EKC/KEOPS complex, composed of PRPK, TPRKB, OSGEP, LAGE3, and GON7. Disease mutations in PRPK and TPRKB are mapped into the structure, and we show that one mutation, PRPK K238Nfs*2, lost the binding to OSGEP. Our structure also makes the virtual screening possible and paves the way for more rational drug design.

Crystal structure of the human PRPK-TPRKB complex.,Li J, Ma X, Banerjee S, Chen H, Ma W, Bode AM, Dong Z Commun Biol. 2021 Feb 5;4(1):167. doi: 10.1038/s42003-021-01683-4. PMID:33547416<ref>PMID:33547416</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6wqx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Banerjee S]]
[[Category: Dong ZG]]
[[Category: Li J]]
[[Category: Ma XL]]