View source for 6xfl


==Structural characterization of the type III secretion system pilotin-secretin complex InvH-InvG by NMR spectroscopy==
<StructureSection load='6xfl' size='340' side='right'caption='[[6xfl]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xfl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XFL FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfl OCA], [https://pdbe.org/6xfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xfl RCSB], [https://www.ebi.ac.uk/pdbsum/6xfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SCTG_SALTY SCTG_SALTY] Involved in the synthesis of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:9680224, PubMed:9786184). Pilot protein that is required for the proper localization of the secretin InvG/SctC in the outer membrane (PubMed:9680224, PubMed:9786184). Required for the secretion of the Sip virulence factors (PubMed:9680224, PubMed:23159244).<ref>PMID:23159244</ref> <ref>PMID:9680224</ref> <ref>PMID:9786184</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The type III secretion system (T3SS) is a multi-membrane-spanning protein channel used by Gram-negative pathogenic bacteria to secrete effectors directly into the host cell cytoplasm. In the many species reliant on the T3SS for pathogenicity, proper assembly of the outer membrane secretin pore depends on a diverse family of lipoproteins called pilotins. We present structural and biochemical data on the Salmonella enterica pilotin InvH and the S domain of its cognate secretin InvG. Characterization of InvH by X-ray crystallography revealed a dimerized, alpha-helical pilotin. Size-exclusion-coupled multi-angle light scattering and small-angle X-ray scattering provide supporting evidence for the formation of an InvH homodimer in solution. Structures of the InvH-InvG heterodimeric complex determined by X-ray crystallography and NMR spectroscopy indicate a predominantly hydrophobic interface. Knowledge of the interaction between InvH and InvG brings us closer to understanding the mechanisms by which pilotins assemble the secretin pore.

Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods.,Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ Structure. 2020 Aug 27. pii: S0969-2126(20)30288-4. doi:, 10.1016/j.str.2020.08.006. PMID:32877645<ref>PMID:32877645</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xfl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
[[Category: Heinkel F]]
[[Category: Majewski DD]]
[[Category: McIntosh LP]]
[[Category: Okon M]]
[[Category: Robb CS]]
[[Category: Strynadka NCJ]]
[[Category: Vuckovic M]]