View source for 6xhq


==Crystal structure of S. aureus TarI in complex with CDP-ribitol (space group C121)==
<StructureSection load='6xhq' size='340' side='right'caption='[[6xhq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xhq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XHQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=V2V:CDP-ribitol'>V2V</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xhq OCA], [https://pdbe.org/6xhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xhq RCSB], [https://www.ebi.ac.uk/pdbsum/6xhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xhq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TARI1_STAA8 TARI1_STAA8] Catalyzes the transfer of the cytidylyl group of CTP to D-ribitol 5-phosphate.[HAMAP-Rule:MF_02068]<ref>PMID:15362865</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cell wall of many pathogenic Gram-positive bacteria contains ribitol-phosphate wall teichoic acid (WTA), a polymer that is linked to virulence and regulation of essential physiological processes including cell division. CDP-ribitol, the activated precursor for ribitol-phosphate polymerization, is synthesized by a cytidylyltransferase and reductase pair known as TarI and TarJ, respectively. In this study, we present crystal structures of Staphylococcus aureus TarI and TarJ in their apo forms and in complex with substrates and products. The TarI structures illustrate the mechanism of CDP-ribitol synthesis from CTP and ribitol-phosphate and reveal structural changes required for substrate binding and catalysis. Insights into the upstream step of ribulose-phosphate reduction to ribitol-phosphate is provided by the structures of TarJ. Furthermore, we propose a general topology of the enzymes in a heterotetrameric form built using restraints from crosslinking mass spectrometry analysis. Together, our data present molecular details of CDP-ribitol production that may aid in the design of inhibitors against WTA biosynthesis.

Crystallographic analysis of TarI and TarJ, a cytidylyltransferase and reductase pair for CDP-ribitol synthesis in Staphylococcus aureus wall teichoic acid biogenesis.,Li FKK, Gale RT, Petrotchenko EV, Borchers CH, Brown ED, Strynadka NCJ J Struct Biol. 2021 Apr 2:107733. doi: 10.1016/j.jsb.2021.107733. PMID:33819634<ref>PMID:33819634</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xhq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Li FKK]]
[[Category: Strynadka NCJ]]