View source for 6xio


==ADP-dependent kinase complex with fructose-6-phosphate and ADPbetaS==
<StructureSection load='6xio' size='340' side='right'caption='[[6xio]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcinales_archaeon Methanosarcinales archaeon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XIO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.12&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AT4:5-O-[(R)-HYDROXY(THIOPHOSPHONOOXY)PHOSPHORYL]ADENOSINE'>AT4</scene>, <scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xio OCA], [https://pdbe.org/6xio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xio RCSB], [https://www.ebi.ac.uk/pdbsum/6xio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xio ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
ADP-dependent kinases were first described in archaea, although their presence has also been reported in bacteria and eukaryotes (human and mouse). This enzyme family comprises three substrate specificities; specific phosphofructokinases (ADP-PFKs), specific glucokinases (ADP-GKs), and bifunctional enzymes (ADP-PFK/GK). Although many structures are available for members of this family, none exhibits fructose-6-phosphate (F6P) at the active site. Using an ancestral enzyme, we obtain the first structure of an ADP-dependent kinase (AncMsPFK) with F6P at its active site. Key residues for sugar binding and catalysis were identified by alanine scanning, D36 being a critical residue for F6P binding and catalysis. However, this residue hinders glucose binding because its mutation to alanine converts the AncMsPFK enzyme into a specific ADP-GK. Residue K179 is critical for F6P binding, while residues N181 and R212 are also important for this sugar binding, but to a lesser extent. This structure also provides evidence for the requirement of both substrates (sugar and nucleotide) to accomplish the conformational change leading to a closed conformation. This suggests that AncMsPFK mainly populates two states (open and closed) during the catalytic cycle, as reported for specific ADP-PFK. This situation differs from that described for specific ADP-GK enzymes, where each substrate independently causes a sequential domain closure, resulting in three conformational states (open, semiclosed, and closed).

Structure of an ancestral ADP-dependent kinase with fructose-6P reveals key residues for binding, catalysis, and ligand-induced conformational changes.,Munoz SM, Castro-Fernandez V, Guixe V J Biol Chem. 2020 Dec 24;296:100219. doi: 10.1074/jbc.RA120.015376. PMID:33839685<ref>PMID:33839685</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6xio" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phosphofructokinase 3D structures|Phosphofructokinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanosarcinales archaeon]]
[[Category: Castro-Fernandez V]]
[[Category: Fuentes N]]
[[Category: Gonzalez-Ordenes F]]
[[Category: Herrera-Morande A]]
[[Category: Maturana P]]
[[Category: Munoz S]]
[[Category: Villalobos P]]