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==Cryo-EM structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with PIP2==
<StructureSection load='6xit' size='340' side='right'caption='[[6xit]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XIT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xit OCA], [https://pdbe.org/6xit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xit RCSB], [https://www.ebi.ac.uk/pdbsum/6xit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xit ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
G-protein-gated inward rectifier potassium (GIRK) channels are regulated by G proteins and PIP2. Here, using cryo-EM single particle analysis we describe the equilibrium ensemble of structures of neuronal GIRK2 as a function of the C8-PIP2 concentration. We find that PIP2 shifts the equilibrium between two distinguishable structures of neuronal GIRK (GIRK2), extended and docked, towards the docked form. In the docked form the cytoplasmic domain, to which Gbetagamma binds, becomes accessible to the cytoplasmic membrane surface where Gbetagamma resides. Furthermore, PIP2 binding reshapes the Gbetagamma binding surface on the cytoplasmic domain, preparing it to receive Gbetagamma. We find that cardiac GIRK (GIRK1/4) can also exist in both extended and docked conformations. These findings lead us to conclude that PIP2 influences GIRK channels in a structurally similar manner to Kir2.2 channels. In Kir2.2 channels, the PIP2-induced conformational changes open the pore. In GIRK channels, they prepare the channel for activation by Gbetagamma.

Cryo-EM analysis of PIP2 regulation in mammalian GIRK channels.,Niu Y, Tao X, Touhara KK, MacKinnon R Elife. 2020 Aug 26;9. pii: 60552. doi: 10.7554/eLife.60552. PMID:32844743<ref>PMID:32844743</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6xit" style="background-color:#fffaf0;"></div>

==See Also==
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: MacKinnon R]]
[[Category: Niu Y]]
[[Category: Tao X]]