View source for 6xix


==Triuret Hydrolase (TrtA) from Herbaspirillum sp. BH-1==
<StructureSection load='6xix' size='340' side='right'caption='[[6xix]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xix]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Herbaspirillum_sp._BH-1 Herbaspirillum sp. BH-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XIX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xix OCA], [https://pdbe.org/6xix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xix RCSB], [https://www.ebi.ac.uk/pdbsum/6xix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xix ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2N6JFX7_9BURK A0A2N6JFX7_9BURK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Triuret (carbonyldiurea) is an impurity found in industrial urea fertilizer (&lt;0.1% w/w) that is applied, worldwide, around 300 million pounds each year on agricultural lands. In addition to anthropogenic sources, endogenous triuret has been identified in amoeba and human urine, the latter being diagnostic for hypokalemia. The present study is the first to describe the metabolic breakdown of triuret, which funnels into biuret metabolism. We identified the gene responsible for triuret decomposition (trtA) in bacterial genomes, clustered with biuH, that encodes biuret hydrolase and has close protein sequence homology. TrtA is a member of the isochorismatase-like hydrolase protein family (IHL), similarly to BiuH, and has a catalytic efficiency (kcat/KM) of 6 x 10(5) (M(-1)s(-1)), a KM for triuret of 20 muM, and exquisite substrate specificity. Indeed, TrtA has four orders of magnitude less activity with biuret. Crystal structures of TrtA in apo and holo form were solved and compared to the BiuH structure. The high substrate selectivity was found to be conveyed by second shell residues around each active site. Mutagenesis of residues conserved in TrtA to the alternate consensus found in BiuHs revealed residues critical to triuret hydrolase activity but no single mutant evolved more biuret activity and likely a combination of mutations is required to interconvert between TrtA, BiuH functions. TrtA-mediated triuret metabolism is relatively rare in recorded genomes (1-2%), but is largely found in plant-associated, nodulating and endophytic bacteria. This study suggests functions for triuret hydrolase in certain eukaryotic intermediary processes and prokaryotic intermediary or biodegradative metabolism.

Discovery of an ultra-specific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway.,Tassoulas LJ, Elias MH, Wackett LP J Biol Chem. 2020 Nov 10. pii: RA120.015631. doi: 10.1074/jbc.RA120.015631. PMID:33172891<ref>PMID:33172891</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xix" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Herbaspirillum sp. BH-1]]
[[Category: Large Structures]]
[[Category: Elias MH]]
[[Category: Tassoulas LT]]
[[Category: Wackett LP]]