View source for 6xjm


==Biuret Hydrolase (BiuH) from Rhodococcus sp. Mel C169S bound with biuret==
<StructureSection load='6xjm' size='340' side='right'caption='[[6xjm]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xjm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp._Mel Rhodococcus sp. Mel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5J:dicarbonimidic+diamide'>C5J</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xjm OCA], [https://pdbe.org/6xjm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xjm RCSB], [https://www.ebi.ac.uk/pdbsum/6xjm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xjm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H8ZKV9_9NOCA H8ZKV9_9NOCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Triuret (carbonyldiurea) is an impurity found in industrial urea fertilizer (&lt;0.1% w/w) that is applied, worldwide, around 300 million pounds each year on agricultural lands. In addition to anthropogenic sources, endogenous triuret has been identified in amoeba and human urine, the latter being diagnostic for hypokalemia. The present study is the first to describe the metabolic breakdown of triuret, which funnels into biuret metabolism. We identified the gene responsible for triuret decomposition (trtA) in bacterial genomes, clustered with biuH, that encodes biuret hydrolase and has close protein sequence homology. TrtA is a member of the isochorismatase-like hydrolase protein family (IHL), similarly to BiuH, and has a catalytic efficiency (kcat/KM) of 6 x 10(5) (M(-1)s(-1)), a KM for triuret of 20 muM, and exquisite substrate specificity. Indeed, TrtA has four orders of magnitude less activity with biuret. Crystal structures of TrtA in apo and holo form were solved and compared to the BiuH structure. The high substrate selectivity was found to be conveyed by second shell residues around each active site. Mutagenesis of residues conserved in TrtA to the alternate consensus found in BiuHs revealed residues critical to triuret hydrolase activity but no single mutant evolved more biuret activity and likely a combination of mutations is required to interconvert between TrtA, BiuH functions. TrtA-mediated triuret metabolism is relatively rare in recorded genomes (1-2%), but is largely found in plant-associated, nodulating and endophytic bacteria. This study suggests functions for triuret hydrolase in certain eukaryotic intermediary processes and prokaryotic intermediary or biodegradative metabolism.

Discovery of an ultra-specific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway.,Tassoulas LJ, Elias MH, Wackett LP J Biol Chem. 2020 Nov 10. pii: RA120.015631. doi: 10.1074/jbc.RA120.015631. PMID:33172891<ref>PMID:33172891</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xjm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodococcus sp. Mel]]
[[Category: Elias MH]]
[[Category: Tassoulas LT]]
[[Category: Wackett LP]]