View source for 6xwy


==Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed==
<StructureSection load='6xwy' size='340' side='right'caption='[[6xwy]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xwy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XWY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwy OCA], [https://pdbe.org/6xwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xwy RCSB], [https://www.ebi.ac.uk/pdbsum/6xwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Engineered proteins must be phenotypically selected for function in the appropriate physiological context. Here, we present a versatile approach that allows generating panels of mammalian cells that express diversified heterologous protein libraries in the cytosol or subcellular compartments under stable conditions and in a single-variant-per-cell manner. To this end we adapt CRISPR/Cas9 editing technology to diversify targeted stretches of a protein of interest in situ. We demonstrate the utility of the approach by in situ engineering and intra-lysosome specific selection of an extremely pH-resistant long Stokes shift red fluorescent protein variant. Tailoring properties to specific conditions of cellular sub-compartments or organelles of mammalian cells can be an important asset to optimize various proteins, protein-based tools, and biosensors for distinct functions.

Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.,Erdogan M, Fabritius A, Basquin J, Griesbeck O Cell Chem Biol. 2020 Mar 3. pii: S2451-9456(20)30068-4. doi:, 10.1016/j.chembiol.2020.02.004. PMID:32142629<ref>PMID:32142629</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xwy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Entacmaea quadricolor]]
[[Category: Large Structures]]
[[Category: Basquin J]]
[[Category: Erdogan M]]
[[Category: Fabritius A]]
[[Category: Griesbeck O]]