View source for 6xzc


==CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis==
<StructureSection load='6xzc' size='340' side='right'caption='[[6xzc]], [[Resolution|resolution]] 3.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6xzc]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XZC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.37&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzc OCA], [https://pdbe.org/6xzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xzc RCSB], [https://www.ebi.ac.uk/pdbsum/6xzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ESPB_MYCTU ESPB_MYCTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The EspB protein of Mycobacterium tuberculosis is a 60 kDa virulence factor, implicated in conjugation and exported by the ESX-1 system of which it may also be a component. Previous attempts to obtain high-resolution maps of EspB by cryo-electron microscopic examination of single particles have been thwarted by severe orientation bias of the particles. This was overcome by using detergent as a surfactant thereby allowing reconstruction of the EspB structure at 3.37 A resolution. The final structure revealed the N-terminal domain of EspB to be organized as a cylindrical heptamer with dimensions of 90 A x 90 A and a central channel of 45 A diameter whereas the C-terminal domain was unstructured. New atomic insight was obtained into the helical packing required for protomer interactions and the overall electrostatic potential. The external surface is electronegatively charged while the channel is lined with electropositive patches. EspB thus has many features of a pore-like transport protein that might allow the passage of an ESX-1 substrate such as the 35 A diameter EsxA-EsxB heterodimer or B-form DNA consistent with its proposed role in DNA uptake.

High resolution CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis.,Piton J, Pojer F, Wakatsuki S, Gati C, Cole ST J Struct Biol X. 2020 Jul 2;4:100029. doi: 10.1016/j.yjsbx.2020.100029., eCollection 2020. PMID:32875288<ref>PMID:32875288</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xzc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Cole ST]]
[[Category: Gati C]]
[[Category: Piton J]]
[[Category: Pojer F]]
[[Category: Wakatsuki S]]