View source for 6yao


==Crystal structure of ZmCKO4a in complex with inhibitor 1-[2-(2-Hydroxy-ethyl)-phenyl]-3-(3-trifluoromethoxy-phenyl)-urea==
<StructureSection load='6yao' size='340' side='right'caption='[[6yao]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YAO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=OJ2:1-[2-(2-Hydroxy-ethyl)-phenyl]-3-(3-trifluoromethoxy-phenyl)-urea'>OJ2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yao OCA], [https://pdbe.org/6yao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yao RCSB], [https://www.ebi.ac.uk/pdbsum/6yao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yao ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Increasing crop productivity is our major challenge if we are to meet global needs for food, feed and fuel. Controlling the level of the plant hormones cytokinins is a mean of improving plant productivity. Cytokinin oxidase/dehydrogenase (CKO/CKX) is a major target in this regard because it degrades cytokinins. Here, we describe the synthesis and biological activities of new CKX inhibitors derived mainly from diphenylurea. They were tested on four CKX isoforms from maize and Arabidopsis, where the best compounds showed IC50 values in the 10 -8 M concentration range. The binding mode of the most efficient inhibitors was characterized from high-resolution crystal complexed structures. Although these compounds do not possess intrinsic cytokinin activity, we have demonstrated their tremendous potential for use in the plant tissue culture industry as well as in agriculture. We have identified a key substance, compound 19, which increases stress resistance and seed yield in Arabidopsis, but also improves the yield of wheat, barley and rapeseed grains under field conditions. Thus our findings reveal that subtle modulation of cytokinin levels via CKX inhibition can positively affect plant growth, development and yield, and underline the fact that CKX inhibitors truly deserve to attract interest in plant biotechnology and agriculture.

Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture.,Nisler J, Kopecny D, Pekna Z, Koncitikova R, Koprna R, Murvanidze N, Werbrouck SPO, Havlicek L, De Diego N, Kopecna M, Wimmer Z, Briozzo P, Morera S, Zalabak D, Spichal L, Strnad M J Exp Bot. 2020 Sep 18. pii: 5908322. doi: 10.1093/jxb/eraa437. PMID:32945834<ref>PMID:32945834</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6yao" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Briozzo P]]
[[Category: Kopecny D]]
[[Category: Morera S]]