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==Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of ADP==
<StructureSection load='6yay' size='340' side='right'caption='[[6yay]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6yay]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YAY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yay OCA], [https://pdbe.org/6yay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yay RCSB], [https://www.ebi.ac.uk/pdbsum/6yay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yay ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BCSQ_ECOLI BCSQ_ECOLI] Essential for cellulose biosynthesis, shown for strain 1094, a commensal, natural cellulose producer (PubMed:19400787). Also shown in strain W3110 which has a restored reading frame (TAG stop codon to TTG for amino acid 6, called strain AR3110), this protein (PubMed:24097954). May play a role in subcellular localization of an active cellulose biosynthesis apparatus at the bacterial cell pole (PubMed:19400787). The combination of cellulose and the curli fiber network confer cohesion, elasticity and tissue-like properties to colonies (PubMed:24097954).<ref>PMID:19400787</ref> <ref>PMID:24097954</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.

Architecture and regulation of an enterobacterial cellulose secretion system.,Abidi W, Zouhir S, Caleechurn M, Roche S, Krasteva PV Sci Adv. 2021 Jan 27;7(5). pii: 7/5/eabd8049. doi: 10.1126/sciadv.abd8049. Print , 2021 Jan. PMID:33563593<ref>PMID:33563593</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6yay" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Abidi W]]
[[Category: Caleechurn M]]
[[Category: Krasteva PV]]
[[Category: Roche S]]
[[Category: Zouhir S]]