View source for 6ylx


==pre-60S State NE1 (TAP-Flag-Nop53)==
<StructureSection load='6ylx' size='340' side='right'caption='[[6ylx]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ylx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YLX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ylx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ylx OCA], [https://pdbe.org/6ylx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ylx RCSB], [https://www.ebi.ac.uk/pdbsum/6ylx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ylx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL3_YEAST RL3_YEAST] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.

Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.,Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E Mol Cell. 2020 Aug 20;79(4):615-628.e5. doi: 10.1016/j.molcel.2020.06.032. Epub, 2020 Jul 14. PMID:32668200<ref>PMID:32668200</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ylx" style="background-color:#fffaf0;"></div>

==See Also==
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Beckmann R]]
[[Category: Kater L]]