View source for 6ymz


==Structure of the CheB methylsterase from P. atrosepticum SCRI1043==
<StructureSection load='6ymz' size='340' side='right'caption='[[6ymz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ymz]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_atrosepticum_SCRI1043 Pectobacterium atrosepticum SCRI1043]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YMZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ymz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ymz OCA], [https://pdbe.org/6ymz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ymz RCSB], [https://www.ebi.ac.uk/pdbsum/6ymz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ymz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHEB_PECAS CHEB_PECAS] Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid (By similarity). Does not interact with the C-terminal pentapeptide of the chemoreceptors (PubMed:33187094).[HAMAP-Rule:MF_00099]<ref>PMID:33187094</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many bacteria possess multiple chemosensory pathways that are composed of homologous signaling proteins. These pathways appear to be functionally insulated from each other, but little information is available on the corresponding molecular basis. We report here a novel mechanism that contributes to pathway insulation. We show that, of the four CheB paralogs of Pseudomonas aeruginosa PAO1, only CheB2 recognizes a pentapeptide at the C-terminal extension of the McpB (Aer2) chemoreceptor (KD = 93 microM). McpB is the sole chemoreceptor that stimulates the Che2 pathway, and CheB2 is the methylesterase of this pathway. Pectobacterium atrosepticum SCRI1043 has a single CheB, CheB_Pec, and 19 of its 36 chemoreceptors contain a C-terminal pentapeptide. The deletion of cheB_Pec abolished chemotaxis, but, surprisingly, none of the pentapeptides bound to CheB_Pec. To determine the corresponding structural basis, we solved the 3D structure of CheB_Pec. Its structure aligned well with that of the pentapeptide-dependent enzyme from Salmonella enterica. However, no electron density was observed in the CheB_Pec region corresponding to the pentapeptide-binding site in the Escherichia coli CheB. We hypothesize that this structural disorder is associated with the failure to bind pentapeptides. Combined data show that CheB methylesterases can be divided into pentapeptide-dependent and independent enzymes.

Evidence for Pentapeptide-Dependent and Independent CheB Methylesterases.,Velando F, Gavira JA, Rico-Jimenez M, Matilla MA, Krell T Int J Mol Sci. 2020 Nov 11;21(22). pii: ijms21228459. doi: 10.3390/ijms21228459. PMID:33187094<ref>PMID:33187094</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ymz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pectobacterium atrosepticum SCRI1043]]
[[Category: Gavira JA]]
[[Category: Krell T]]
[[Category: Matilla MA]]
[[Category: Velando-Soriano F]]