View source for 6z70


==Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state==
<StructureSection load='6z70' size='340' side='right'caption='[[6z70]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6z70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z70 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z70 OCA], [https://pdbe.org/6z70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z70 RCSB], [https://www.ebi.ac.uk/pdbsum/6z70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z70 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O66528_AQUAE O66528_AQUAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multidrug and toxic compound extrusion (MATE) transporters are widespread in all domains of life. Bacterial MATE transporters confer multidrug resistance by utilizing an electrochemical gradient of H(+) or Na(+) to export xenobiotics across the membrane. Despite the availability of X-ray structures of several MATE transporters, a detailed understanding of the transport mechanism has remained elusive. Here we report the crystal structure of a MATE transporter from Aquifex aeolicus at 2.0-A resolution. In light of its phylogenetic placement outside of the diversity of hitherto-described MATE transporters and the lack of conserved acidic residues, this protein may represent a subfamily of prokaryotic MATE transporters, which was proven by phylogenetic analysis. Furthermore, the crystal structure and substrate docking results indicate that the substrate binding site is located in the N bundle. The importance of residues surrounding this binding site was demonstrated by structure-based site-directed mutagenesis. We suggest that Aq_128 is functionally similar but structurally diverse from DinF subfamily transporters. Our results provide structural insights into the MATE transporter, which further advances our global understanding of this important transporter family.

The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.,Zhao J, Xie H, Mehdipour AR, Safarian S, Ermler U, Munke C, Thielmann Y, Hummer G, Ebersberger I, Wang J, Michel H Proc Natl Acad Sci U S A. 2021 Nov 16;118(46). pii: 2107335118. doi:, 10.1073/pnas.2107335118. PMID:34753818<ref>PMID:34753818</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6z70" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus VF5]]
[[Category: Large Structures]]
[[Category: Michel H]]
[[Category: Safarian S]]
[[Category: Thielmann Y]]
[[Category: Wang J]]
[[Category: Xie H]]
[[Category: Zhao J]]