View source for 6zdp


==Structure of telomerase from Candida Tropicalis in complexe with TWJ fragment of telomeric RNA==
<StructureSection load='6zdp' size='340' side='right'caption='[[6zdp]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6zdp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_tropicalis_MYA-3404 Candida tropicalis MYA-3404]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZDP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zdp OCA], [https://pdbe.org/6zdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zdp RCSB], [https://www.ebi.ac.uk/pdbsum/6zdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zdp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C5MCQ7_CANTT C5MCQ7_CANTT] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.[RuleBase:RU365061]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Telomerase plays critical roles in cellular aging, in the emergence and/or development of cancer, and in the capacity for stem-cell renewal, consists of a catalytic telomerase reverse transcriptase (TERT) and a template-encoding RNA (TER). TERs from diverse organisms contain two conserved structural elements: the template-pseudoknot (T-PK) and a helical three-way junction (TWJ). Species-specific features of the structure and function of telomerase make obtaining a more in-depth understanding of the molecular mechanism of telomerase particularly important. Here, we report the first structural studies of N-terminally truncated TERTs from Candida albicans and Candida tropicalis in apo form and complexed with their respective TWJs in several conformations. We found that Candida TERT proteins perform only one round of telomere addition in the presence or absence of PK/TWJ and display standard reverse transcriptase activity. The C-terminal domain adopts at least two extreme conformations and undergoes conformational interconversion, which regulates the catalytic activity. Most importantly, we identified a conserved tertiary structural motif, called the U-motif, which interacts with the reverse transcriptase domain and is crucial for catalytic activity. Together these results shed new light on the structure and mechanics of fungal TERTs, which show common TERT characteristics, but also display species-specific features.

Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungidouble dagger.,Zhai LT, Rety S, Chen WF, Song ZY, Auguin D, Sun B, Dou SX, Xi XG Nucleic Acids Res. 2021 Apr 15. pii: 6226674. doi: 10.1093/nar/gkab261. PMID:33856462<ref>PMID:33856462</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6zdp" style="background-color:#fffaf0;"></div>

==See Also==
*[[Telomerase 3D structures|Telomerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candida tropicalis MYA-3404]]
[[Category: Large Structures]]
[[Category: Auguin D]]
[[Category: Chen WF]]
[[Category: Rety S]]
[[Category: Xi XG]]
[[Category: Zhai L]]