View source for 6zdx


==RIFIN variable region bound to LILRB1 ectodomain==
<StructureSection load='6zdx' size='340' side='right'caption='[[6zdx]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6zdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZDX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zdx OCA], [https://pdbe.org/6zdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zdx RCSB], [https://www.ebi.ac.uk/pdbsum/6zdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zdx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8I4N9_PLAF7 Q8I4N9_PLAF7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Plasmodium species that cause malaria are obligate intracellular parasites, and disease symptoms occur as they replicate within human blood. Despite risking immune detection, the parasite delivers proteins that bind host receptors to infected erythrocyte surfaces. In the causative agent of the most deadly human malaria, Plasmodium falciparum, RIFINs form the largest erythrocyte surface protein family(1). Some RIFINs can bind inhibitory immune receptors, acting as targets for unusual antibodies containing a LAIR1 ectodomain(2-4), or as ligands for LILRB1(5). RIFINs stimulate LILRB1 activation and signalling(5), thereby potentially dampening human immune responses. To understand this process, we determined a structure of a RIFIN bound to LILRB1. We show that the RIFIN mimics the natural activating ligand of LILRB1, MHC class I, in its LILRB1-binding mode. A single RIFIN mutation disrupts the complex, blocks LILRB1 binding by all tested RIFINs and abolishes signalling in a reporter assay. In a supported lipid bilayer system, which mimics NK cell activation by antibody-dependent cell-mediated cytotoxicity, both RIFIN and MHC are recruited to the NK cell immunological synapse and reduce cell activation, as measured by perforin mobilisation. Therefore, LILRB1-binding RIFINs mimic the binding mode of the natural ligand of LILRB1 and suppress NK cell function.

Structural basis for RIFIN-mediated activation of LILRB1 in malaria.,Harrison TE, Morch AM, Felce JH, Sakoguchi A, Reid AJ, Arase H, Dustin ML, Higgins MK Nature. 2020 Jul 10. pii: 10.1038/s41586-020-2530-3. doi:, 10.1038/s41586-020-2530-3. PMID:32650338<ref>PMID:32650338</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6zdx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Leukocyte immunoglobulin-like receptor|Leukocyte immunoglobulin-like receptor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Plasmodium falciparum 3D7]]
[[Category: Harrison TE]]
[[Category: Higgins MK]]