View source for 6zja


==Helicobacter pylori urease with inhibitor bound in the active site==
<StructureSection load='6zja' size='340' side='right'caption='[[6zja]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6zja]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DJM:2-[1-(3,5-dimethylphenyl)imidazol-2-yl]sulfanyl-~{N}-oxidanyl-ethanamide'>DJM</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zja OCA], [https://pdbe.org/6zja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zja RCSB], [https://www.ebi.ac.uk/pdbsum/6zja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zja ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/URE23_HELPY URE23_HELPY] Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.<ref>PMID:8039935</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Infection of the human stomach by Helicobacter pylori remains a worldwide problem and greatly contributes to peptic ulcer disease and gastric cancer. Without active intervention approximately 50% of the world population will continue to be infected with this gastric pathogen. Current eradication, called triple therapy, entails a proton-pump inhibitor and two broadband antibiotics, however resistance to either clarithromycin or metronidazole is greater than 25% and rising. Therefore, there is an urgent need for a targeted, high-specificity eradication drug. Gastric infection by H. pylori depends on the expression of a nickel-dependent urease in the cytoplasm of the bacteria. Here, we report the 2.0 A resolution structure of the 1.1 MDa urease in complex with an inhibitor by cryo-electron microscopy and compare it to a beta-mercaptoethanol-inhibited structure at 2.5 A resolution. The structural information is of sufficient detail to aid in the development of inhibitors with high specificity and affinity.

Cryo-EM structure of Helicobacter pylori urease with an inhibitor in the active site at 2.0 A resolution.,Cunha ES, Chen X, Sanz-Gaitero M, Mills DJ, Luecke H Nat Commun. 2021 Jan 11;12(1):230. doi: 10.1038/s41467-020-20485-6. PMID:33431861<ref>PMID:33431861</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6zja" style="background-color:#fffaf0;"></div>

==See Also==
*[[Urease 3D structures|Urease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Helicobacter pylori]]
[[Category: Large Structures]]
[[Category: Cunha E]]
[[Category: Luecke H]]