View source for 6zjc


==Crystal structure of Equus ferus caballus glutathione transferase A3-3 in complex with glutathione and triethyltin==
<StructureSection load='6zjc' size='340' side='right'caption='[[6zjc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=QLT:Triethyltin+bromide'>QLT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zjc OCA], [https://pdbe.org/6zjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zjc RCSB], [https://www.ebi.ac.uk/pdbsum/6zjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zjc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Organotin compounds are highly toxic environmental pollutants with neurotoxic and endocrine-disrupting effects. They are potent inhibitors of glutathione transferases (GSTs), thus impeding their detoxication and antioxidant functions. Several GSTs, including equine GST A3-3 (EcaGST A3-3), exhibit steroid double-bond isomerase activity and are involved in the biosynthesis of testosterone and progesterone. We have performed enzyme kinetics analyses of the inhibition of EcaGST A3-3 by organotin compounds. We have also solved crystal structures of EcaGST A3-3 in complexes with glutathione, and with glutathione together with covalently bound triethyltin. Our structural data indicate that the tin atom forms strong bonds with a covalent character not only with the glutathione, but also with a tyrosyl residue of the enzyme itself, thereby preventing the release of the glutathione-organotin adduct and completely blocking the enzyme function. This work presents a structural basis for the general mechanism of GST inhibition by organotin compounds and contributes to the understanding of their neurotoxic and endocrine disrupting effects.

Structural and functional analysis of the inhibition of equine glutathione transferase A3-3 by organotin endocrine disrupting pollutants.,Skerlova J, Ismail A, Lindstrom H, Sjodin B, Mannervik B, Stenmark P Environ Pollut. 2020 Oct 29:115960. doi: 10.1016/j.envpol.2020.115960. PMID:33162212<ref>PMID:33162212</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6zjc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ismail A]]
[[Category: Lindstrom H]]
[[Category: Mannervik B]]
[[Category: Sjodin B]]
[[Category: Skerlova J]]
[[Category: Stenmark P]]