View source for 7a6p


==Structural determinants underlying the adduct lifetime in a short LOV protein PpSB2-LOV==
<StructureSection load='7a6p' size='340' side='right'caption='[[7a6p]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7a6p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A6P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a6p OCA], [https://pdbe.org/7a6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a6p RCSB], [https://www.ebi.ac.uk/pdbsum/7a6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a6p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q88JB0_PSEPK Q88JB0_PSEPK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The primary photochemistry is similar among the flavin-bound sensory domains of LOV (Light-oxygen-voltage) photoreceptors, where upon blue-light illumination a covalent adduct is formed on the microseconds time scale between the flavin chromophore and a strictly conserved cysteine residue. In contrast, the adduct-state decay kinetics vary from seconds to days or longer. The molecular basis for this variation among structurally conserved LOV domains is not fully understood. Here we selected PpSB2-LOV, a fast cycling (taurec 3.5 min, 20 degrees C) short LOV protein from Pseudomonas putida that shares 67% sequence identity with a slow-cycling (taurec 2467 min, 20 degrees C) homologous protein PpSB1-LOV. Based on the crystal structure of the PpSB2-LOV in the dark state reported here, we used a comparative approach, in which we combined structure and sequence information with molecular dynamics (MD) simulations to address the mechanistic basis for the vastly different adduct-state lifetimes in the two homologous proteins. MD simulations pointed towards dynamically distinct structural region, which were subsequently targeted by site-directed mutagenesis of PpSB2-LOV, where we introduced single- and multi-site substitutions exchanging them with the corresponding residues from PpSB1-LOV. Collectively, the data presented identifies key amino acids on the Abeta-Bbeta, Ealpha-Falpha loops, and the Falpha helix, such as E27 and I66, that play a decisive role in determining the adduct-lifetime. Our results additionally suggest a correlation between the solvent-accessibility of the chromophore pocket and adduct-state lifetime. The presented results add to our understanding of LOV signaling, and will have important implications in tuning the signaling behavior (on/off kinetics) of LOV-based optogenetic tools.

Structural determinants underlying the adduct lifetime in the LOV proteins of Pseudomonas putida.,Arinkin V, Granzin J, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R FEBS J. 2021 Feb 23. doi: 10.1111/febs.15785. PMID:33621443<ref>PMID:33621443</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7a6p" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas putida KT2440]]
[[Category: Arinkin V]]
[[Category: Batra-Safferling R]]
[[Category: Granzin J]]