View source for 7ae0


==Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the sheath-tube module in extended state.==
<StructureSection load='7ae0' size='340' side='right'caption='[[7ae0]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ae0]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Algoriphagus_machipongonensis Algoriphagus machipongonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AE0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ae0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ae0 OCA], [https://pdbe.org/7ae0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ae0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ae0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ae0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A3HTC2_9BACT A3HTC2_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). Bioinformatic studies uncovered a phylogenetic group of hundreds of putative CIS gene clusters that are highly diverse and widespread; however, only four systems have been characterized. Here we studied a putative CIS gene cluster in the marine bacterium Algoriphagus machipongonensis. Using an integrative approach, we show that the system is compatible with an eCIS mode of action. Our cryo-electron microscopy structure revealed several features that differ from those seen in other CISs: a 'cap adaptor' located at the distal end, a 'plug' exposed to the tube lumen, and a 'cage' formed by massive extensions of the baseplate. These elements are conserved in other CISs, and our genetic tools identified that they are required for assembly, cargo loading and function. Furthermore, our atomic model highlights specific evolutionary hotspots and will serve as a framework for understanding and re-engineering CISs.

Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.,Xu J, Ericson CF, Lien YW, Rutaganira FUN, Eisenstein F, Feldmuller M, King N, Pilhofer M Nat Microbiol. 2022 Feb 14. pii: 10.1038/s41564-022-01059-2. doi:, 10.1038/s41564-022-01059-2. PMID:35165385<ref>PMID:35165385</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ae0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Algoriphagus machipongonensis]]
[[Category: Large Structures]]
[[Category: Ericson C]]
[[Category: Feldmueller M]]
[[Category: Lien YW]]
[[Category: Pilhofer M]]
[[Category: Xu J]]