View source for 7arc


==Cryo-EM structure of Polytomella Complex-I (peripheral arm)==
<StructureSection load='7arc' size='340' side='right'caption='[[7arc]], [[Resolution|resolution]] 2.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7arc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Polytomella_sp._Pringsheim_198.80 Polytomella sp. Pringsheim 198.80]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ARC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ARC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8Q1:S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]+dodecanethioate'>8Q1</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7arc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7arc OCA], [https://pdbe.org/7arc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7arc RCSB], [https://www.ebi.ac.uk/pdbsum/7arc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7arc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A7S0V8A9_9CHLO A0A7S0V8A9_9CHLO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and the 51-subunit complex I from the green alga Polytomella sp., both at around 2.9 A resolution. In both complexes, a heterotrimeric gamma-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 (NADH dehydrogenase subunit 1) loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We propose that the bridge domain participates in regulating the activity of plant complex I.

A ferredoxin bridge connects the two arms of plant mitochondrial complex I.,Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP Plant Cell. 2021 Jul 19;33(6):2072-2091. doi: 10.1093/plcell/koab092. PMID:33768254<ref>PMID:33768254</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7arc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Polytomella sp. Pringsheim 198 80]]
[[Category: Klusch N]]
[[Category: Kuehlbrandt W]]
[[Category: Yildiz O]]