View source for 7ata


==Nudaurelia capensis omega virus procapsid: virus-like particles expressed in Nicotiana benthamiana==
<SX load='7ata' size='340' side='right' viewer='molstar' caption='[[7ata]], [[Resolution|resolution]] 6.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ata]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Nudaurelia_capensis_omega_virus Nudaurelia capensis omega virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ATA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ATA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.63&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ata FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ata OCA], [https://pdbe.org/7ata PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ata RCSB], [https://www.ebi.ac.uk/pdbsum/7ata PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ata ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q4TVS9_9VIRU Q4TVS9_9VIRU] 
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== Publication Abstract from PubMed ==
Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these remain poorly understood. As a tool for studying virus maturation, we transiently expressed the capsid protein of the insect virus Nudaurelia capensis omega virus (NomegaV) in Nicotiana benthamiana and were able to purify both immature procapsids and mature capsids from infiltrated leaves by varying the expression time. Cryo-EM analysis of the plant-produced procapsids and mature capsids to 6.6 A and 2.7 A resolution, respectively, reveals that in addition to large scale rigid body motions, internal regions of the subunits are extensively remodelled during maturation, creating the active site required for autocatalytic cleavage and infectivity. The mature particles are biologically active in terms of their ability to lyse membranes and have a structure that is essentially identical to authentic virus. The ability to faithfully recapitulate and visualize a complex maturation process in plants, including the autocatalytic cleavage of the capsid protein, has revealed a ~30 A translation-rotation of the subunits during maturation as well as conformational rearrangements in the N and C-terminal helical regions of each subunit.

Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.,Castells-Graells R, Ribeiro JRS, Domitrovic T, Hesketh EL, Scarff CA, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP Commun Biol. 2021 May 24;4(1):619. doi: 10.1038/s42003-021-02134-w. PMID:34031522<ref>PMID:34031522</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7ata" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Nudaurelia capensis omega virus]]
[[Category: Castells-Graells R]]
[[Category: Domitrovic T]]
[[Category: Hesketh EL]]
[[Category: Johnson JE]]
[[Category: Lawson DM]]
[[Category: Lomonossoff GP]]
[[Category: Ranson NA]]
[[Category: Ribeiro JRS]]
[[Category: Scarff CA]]