View source for 7avc


==DoBi scaffold based on PIH1D1 N-terminal domain==
<StructureSection load='7avc' size='340' side='right'caption='[[7avc]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7avc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AVC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7avc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7avc OCA], [https://pdbe.org/7avc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7avc RCSB], [https://www.ebi.ac.uk/pdbsum/7avc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7avc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PIHD1_HUMAN PIHD1_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Engineered small non-antibody protein scaffolds are a promising alternative to antibodies and are especially attractive for use in protein therapeutics and diagnostics. The advantages include smaller size and a more robust, single-domain structural framework with a defined binding surface amenable to mutation. This calls for a more systematic approach in designing new scaffolds suitable for use in one or more methods of directed evolution. We hereby describe a process based on an analysis of protein structures from the Protein Data Bank and their experimental examination. The candidate protein scaffolds were subjected to a thorough screening including computational evaluation of the mutability, and experimental determination of their expression yield in E. coli, solubility, and thermostability. In the next step, we examined several variants of the candidate scaffolds including their wild types and alanine mutants. We proved the applicability of this systematic procedure by selecting a monomeric single-domain human protein with a fold different from previously known scaffolds. The newly developed scaffold, called ProBi (Protein Binder), contains two independently mutable surface patches. We demonstrated its functionality by training it as a binder against human interleukin-10, a medically important cytokine. The procedure yielded scaffold-related variants with nanomolar affinity.

Protein Binder (ProBi) as a New Class of Structurally Robust Non-Antibody Protein Scaffold for Directed Evolution.,Pham PN, Huliciak M, Biedermannova L, Cerny J, Charnavets T, Fuertes G, Herynek S, Kolarova L, Kolenko P, Pavlicek J, Zahradnik J, Mikulecky P, Schneider B Viruses. 2021 Jan 27;13(2). pii: v13020190. doi: 10.3390/v13020190. PMID:33514045<ref>PMID:33514045</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7avc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Kolenko P]]
[[Category: Mikulecky P]]
[[Category: Pavlicek J]]
[[Category: Pham NP]]
[[Category: Schneider B]]