View source for 7awy


==Crystal structure of the computationally designed Scone-R protein==
<StructureSection load='7awy' size='340' side='right'caption='[[7awy]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7awy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awy OCA], [https://pdbe.org/7awy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awy RCSB], [https://www.ebi.ac.uk/pdbsum/7awy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awy ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric beta-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives.

Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.,Mylemans B, Killian T, Vandebroek L, Van Meervelt L, Tame JRH, Parac-Vogt TN, Voet ARD Acta Crystallogr D Struct Biol. 2021 Jul 1;77(Pt 7):933-942. doi:, 10.1107/S2059798321005787. Epub 2021 Jun 18. PMID:34196619<ref>PMID:34196619</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Mylemans B]]
[[Category: Voet ARD]]